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- Title
Time-resolved electrospray mass spectrometry - a brief history.
- Authors
Lento, Cristina; Audette, Gerald F.; Wilson, Derek J.
- Abstract
This review describes the evolution of time-resolved electrospray ionization mass spectrometry (TRESI-MS), a technology that was developed in large part at Western University. TRESI-MS was initially designed to characterize rapid chemical and biochemical reactions occurring on the millisecond time scale without need for a chromophore. Early TRESI-MS setups usually consisted of continuous-flow rapid mixers with a fixed tee for analysis of a single time point, and later adjustable reaction chamber devices allowing for automatic tracking of the reaction over time. Advances in instrumentation design over the years have resulted in improved time resolution, with microfluidic device implementation allowing for coupling to hydrogen−deuterium exchange (HDX) experiments. Areas of application that will be discussed include the investigation of protein folding intermediates, identification of enzyme−substrate intermediates in the pre-steady state, and the use of time-resolved HDX to study the dynamics of weakly structured protein regions. While some limitations still persist with the method, the continued development of TRESI-MS and related approaches paves the way to a promising future and the study of unexplored application areas.
- Subjects
ELECTROSPRAY ionization mass spectrometry; TIME-resolved spectroscopy; CHROMOPHORES; MICROFLUIDIC devices; PROTEIN folding
- Publication
Canadian Journal of Chemistry, 2015, Vol 93, Issue 1, p7
- ISSN
0008-4042
- Publication type
Article
- DOI
10.1139/cjc-2014-0260