We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Nuclear Factor Kappa B Signaling Initiates Early Differentiation of Neural Stem Cells.
- Authors
Zhang, Yonggang; Liu, Jianjun; Yao, Shaohua; Li, Fang; Xin, Lin; Lai, Mowen; Bracchi-Ricard, Valerie; Xu, Hong; Yen, William; Meng, Wentong; Liu, Shu; Yang, Leiting; Karmally, Shaffiat; Liu, Jin; Zhu, Hongyan; Gordon, Jennifer; Khalili, Kamel; Srinivasan, Shanthi; Bethea, John R.; Mo, Xianming
- Abstract
Inflammatory mediators, many of which activate the signaling of nuclear factor kappa B (NFκB), have received increasing attention in the field of neurogenesis. NFκB signaling regulates neurite outgrowth and neural plasticity as well as the proliferation/apoptosis and terminal differentiation of neural stem cells (NSCs). Early neurogenesis from NSCs produces identical progeny through symmetric division and committed daughter cells through asymmetric division. Here, we show that NFκB signaling is required for NSC initial differentiation. The canonical IKKβ/IκBα/p65 pathway is activated during the initial stages of neural differentiation induced by treatment with TNFα or withdrawal of epidermal growth factor/basic fibroblast growth factor. NSC-specific inhibition of NFκB in transgenic mice causes an accumulation of Nestin+/Sox2+/glial fibrillary acidic protein+ NSCs. Inhibition of NFκB signaling in vitro blocks differentiation and asymmetric division and maintains NSCs in an undifferentiated state. The induction of initial differentiation and asymmetry by NFκB signaling occurs through the inhibition of C/EBPβ expression. Our data reveal a novel function of NFκB signaling in early neurogenesis and provide insight into the molecular mechanisms underlying neurodevelopmental disorders and neurodegenerative diseases. S TEM C ELLS 2012;30:510-524
- Subjects
NF-kappa B; DEVELOPMENTAL neurobiology; APOPTOSIS; STEM cells; FIBROBLASTS
- Publication
Stem Cells, 2012, Vol 30, Issue 3, p510
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1002/stem.1006