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- Title
IgM Molecules with and without J Chain in Serum and after Purification, Studied by Ultra-centrifugation, Electrophoresis, and Electron Microscopy.
- Authors
Eskeland, T.; Christensen, T. B.
- Abstract
IgM molecules in pure IgM preparations and in the corresponding sera from three patients with Waldenström's macroglobulinemia have been studied by analytical ultracentrifugation, rate zonal ultracentrifugation, agarose-polyacrylamide gel electrophoresis, and electron microscopy. One of the IgM preparations was without J chain, while the two others contained J chain. The experiments revealed that IgM with J chain consisted of pentameric molecules; whereas in IgM preparations without J chain the dominating molecules were hexamers, but also pentaniers, tetramers, and even smaller molecules were found. In both types of IgM the molecules formed a wide range of aggregates, which were usually more abundant in the purified IgM than in serum. Some of the aggregates were kept together by noncovalent forces only.
- Subjects
IMMUNOGLOBULIN M; IMMUNOGLOBULINS; SERUM; ELECTROPHORESIS; ELECTROCHEMISTRY; ELECTRON microscopy; POLYACRYLAMIDE gel electrophoresis
- Publication
Scandinavian Journal of Immunology, 1975, Vol 4, Issue 3, p217
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1975.tb02620.x