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- Title
A DNA Vaccine Encoding a Fatty Acid-Binding Protein of Clonorchis sinensis Induces Protective Immune Response in Sprague–Dawley Rats.
- Authors
Lee, J.-S.; Kim, I. S.; Sohn, W.-M.; Lee, J.; Yong, T.-S.
- Abstract
Clonorchis sinensis, the Chinese liver fluke, resides chronically in the biliary tract, and fatty acid-binding protein (FABP) is known to play an important role in the intracellular transport of long-chain fatty acids obtained from the host. Although FABP has stimulated considerable interest as a vaccine candidate, the nature of C. sinensis FABP (CsFABP) remains unclear. We investigated the immunogenicity and protective efficacy of a DNA vaccine encoding CsFABP. The intradermal injection of plasmid DNA carrying the CsFABP gene (pcDNA3.1-FABP) into Sprague–Dawley (SD) rats induced both humoural and cellular immune responses. Animals injected with pcDNA3.1-FABP developed FABP-specific antibody, which is dominance of IgG2a in sera. In addition, the DNA vaccine elicited the production of IFN-γ, but not the production of IL-4 in spleen cells stimulated with recombinant FABP. Moreover, pcDNA3.1-FABP induced a significant level of protection, decreased worm burden (40.9%, P < 0.05) in SD rats against C. sinensis metacerariae challenge. These results suggest that pcDNA3.1-FABP induces a typical T helper-1-dominated immune response and it is a good candidate for use in future clonorchiasis vaccination studies.
- Subjects
DNA vaccines; FATTY acid-binding proteins; GENETIC code; LIVER flukes; IMMUNOREGULATION; INTRADERMAL injections; PREVENTIVE medicine
- Publication
Scandinavian Journal of Immunology, 2006, Vol 63, Issue 3, p169
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2006.01721.x