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- Title
A High-Throughput Circular Tumor Cell Sorting Chip with Trapezoidal Cross Section.
- Authors
Lu, Shijie; Ma, Ding; Mi, Xianqiang
- Abstract
Circulating tumor cells are typically found in the peripheral blood of patients, offering a crucial pathway for the early diagnosis and prediction of cancer. Traditional methods for early cancer diagnosis are inefficient and inaccurate, making it difficult to isolate tumor cells from a large number of cells. In this paper, a new spiral microfluidic chip with asymmetric cross-section is proposed for rapid, high-throughput, label-free enrichment of CTCs in peripheral blood. A mold of the desired flow channel structure was prepared and inverted to make a trapezoidal cross-section using a micro-nanotechnology process of 3D printing. After a systematic study of how flow rate, channel width, and particle concentration affect the performance of the device, we utilized the device to simulate cell sorting of 6 μm, 15 μm, and 25 μm PS (Polystyrene) particles, and the separation efficiency and separation purity of 25 μm PS particles reached 98.3% and 96.4%. On this basis, we realize the enrichment of a large number of CTCs in diluted whole blood (5 mL). The results show that the separation efficiency of A549 was 88.9% and the separation purity was 96.4% at a high throughput of 1400 μL/min. In conclusion, we believe that the developed method is relevant for efficient recovery from whole blood and beneficial for future automated clinical analysis.
- Subjects
MICROFLUIDICS; CHANNEL flow; THREE-dimensional printing; EARLY diagnosis; CANCER diagnosis; POLYSTYRENE
- Publication
Sensors (14248220), 2024, Vol 24, Issue 11, p3552
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s24113552