We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Isolation of α-Glucuronidase Enzyme from a Rumen Metagenomic Library.
- Authors
Lee, Charles; Kibblewhite, Rena; Wagschal, Kurt; Li, Ruiping; Orts, William
- Abstract
α-Glucuronidase enzymes play an essential role in the full enzymatic hydrolysis of hemicellulose. Up to this point, all genes encoding α-glucuronidase enzymes have been cloned from individual, pure culture strains. Using a high-throughput screening strategy, we have isolated the first α-glucuronidase gene ( rum630- AG) from a mixed population of microorganisms. The gene was subcloned into a prokaryotic vector, and the enzyme was overexpressed and biochemically characterized. The RUM630-AG enzyme had optimum activity at pH 6.5 and 40 °C. When birchwood xylan was used as substrate, the RUM630-AG functioned synergistically with an endoxylanase enzyme to hydrolyze the substrate.
- Subjects
GLUCURONIDASE; ENZYMES; HYDROLYSIS; GENES; PROKARYOTES; PLANT growing media
- Publication
Protein Journal, 2012, Vol 31, Issue 3, p206
- ISSN
1572-3887
- Publication type
Article
- DOI
10.1007/s10930-012-9391-z