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- Title
High-resolution, long-term characterization of bacterial motility using optical tweezers.
- Authors
Min, Taejin L.; Mears, Patrick J.; Chubiz, Lon M.; Rao, Christopher V.; Golding, Ido; Chemla, Yann R.
- Abstract
We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of ∼100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.
- Subjects
FLUORESCENCE microscopy; ESCHERICHIA coli; MICROSCOPY; BACTERIA; OPTOELECTRONIC devices
- Publication
Nature Methods, 2009, Vol 6, Issue 11, p831
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1380