We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
DDR complex facilitates global association of RNA polymerase V to promoters and evolutionarily young transposons.
- Authors
Zhong, Xuehua; Hale, Christopher J; Law, Julie A; Johnson, Lianna M; Feng, Suhua; Tu, Andy; Jacobsen, Steven E
- Abstract
The plant-specific DNA-dependent RNA polymerase V (Pol V) evolved from Pol II to function in an RNA-directed DNA methylation pathway. Here, we have identified targets of Pol V in Arabidopsis thaliana on a genome-wide scale using ChIP-seq of NRPE1, the largest catalytic subunit of Pol V. We found that Pol V is enriched at promoters and evolutionarily recent transposons. This localization pattern is highly correlated with Pol V-dependent DNA methylation and small RNA accumulation. We also show that genome-wide chromatin association of Pol V is dependent on all members of a putative chromatin-remodeling complex termed DDR. Our study presents a genome-wide view of Pol V occupancy and sheds light on the mechanistic basis of Pol V localization. Furthermore, these findings suggest a role for Pol V and RNA-directed DNA methylation in genome surveillance and in responding to genome evolution.
- Subjects
RNA polymerase V; TRANSPOSONS; DNA methylation; CATALYTIC activity; CHROMATIN
- Publication
Nature Structural & Molecular Biology, 2012, Vol 19, Issue 9, p870
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2354