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- Title
Assignment<FOOTREF>[sup 1] </FOOTREF> of Sptlc2 to murine chromosomeband 12, region E by in situ hybridization.
- Authors
Osuka, S.; Takeda, J.; Nagiec, M. M.; Dickson, R. C.; Hirabayashi, Y.
- Abstract
This article describes an experiment on encoding of a subunit of murine serine chromosomeband 12 region E by Sptlc2 through in situ hybridization. Sptlc2 (formerly called MmLCB2) encodes a subunit of murine serine palmitoyltransferase (SPI, EC 2.3.1.50), which catalyzes the first biosynthetic step of ceramide, a condensation of L-serine and palmitoyl-CoA. SPT is the key enzyme for the biosynthesis of sphingolipids since ceramide serves as a core structure of all of sphingolipids including sphingomyelin and over 390 species of glycosphingolipids. Although Sptlc2 was isolated as a homologue of Sptlci (MmLCB 1), only Sptlc2 contained a sequence homologous to a pyridoxal phosphate binding motif (around Lys377) found in the E. coli 2-amino-3-ketobutyrate CoA ligase enzyme, which seemed to be a part of the catalytic site of SPI.
- Subjects
HUMAN gene mapping; HUMAN chromosomes; FLUORESCENCE in situ hybridization; HUMAN genetics; BIOCHEMISTRY; BIOSYNTHESIS; CERAMIDES
- Publication
Cytogenetics & Cell Genetics, 1998, Vol 82, Issue 3/4, p222
- ISSN
0301-0171
- Publication type
Article
- DOI
10.1159/000015104