We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Integration of proviral DNA into the PDGF β-receptor gene in HTLV-I-infected T-cells results in a novel tyrosine kinase product with transforming activity.
- Authors
Chi, Kenneth D; McPhee, Roderick A; Wagner, Andrew S; Dietz, James J; Pantazis, Panayotis; Goustin, Anton Scott
- Abstract
We have previously shown that noninfected human T-cell lines express the canonical 5.7 kb mRNA coding for the type β platelet-derived growth factor-receptor (PDGF β-receptor), whereas HTLV-I-infected T-cell lines express a novel PDGF β-receptor mRNA of 3.8 kb. In this report, we have extended those studies to molecularly characterize the 3.8 kb PDGF β-receptor mRNA and show that it has resulted from integration of an apparently undeleted HTLV-I provirus into the PDGF β-receptor gene in an orientation enabling expression of a truncated PDGF β-receptor mRNA using the 3′ HTLV-I long terminal repeat as a promoter. Further, NIH3T3 cells transfected with a plasmid containing the truncated PDGF β-receptor ORF plasmid generate colonies in soft agar with more cells per colony than untransfected cells, or cells transfected with the Tax 1 or PDGF-B (c-sis) plasmids. These results indicate that the truncated PDGF β-receptor protein acquires transforming capability and that HTLV-I-induced truncation of PDGF β-receptor may correlate with HTLV-I-associated neoplasia of human T-cells.
- Subjects
PROTEIN-tyrosine kinases; MUTAGENESIS; T cells
- Publication
Oncogene, 1997, Vol 15, Issue 9, p1051
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1201267