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- Title
Isolation and characterization of a novel transcriptional repressor GmERF6 from soybean.
- Authors
Zhai, Y.; Li, J.; Li, X.; Lei, T.; Yan, F.; Zhao, Y.; Li, Y.; Su, L.; Wang, Y.; Wang, Q.
- Abstract
A new ethylene response factor (ERF), GmERF6, was isolated from soybean. Protein sequence alignment of GmERF6 revealed an AP2/ERF domain, two putative nuclear localization signals (NLSs) and an ERF-associated amphiphilic repression (EAR) motif. Real-time quantitative PCR analysis revealed that the expression of GmERF6 was differentially induced in soybean seedlings by drought, salt, cold, salicylic acid, ethylene, abscisic acid and methyl jasmonate. Transient expression experiments demonstrated that GmERF6 functions as a transcriptional repressor to downregulate the transcriptional levels of the reporter gene and repress the activated ability of other transcriptional activator. Transgenic Arabidopsis lines constitutively expressing GmERF6 showed an increased tolerance to drought compared to wild-type plants.
- Subjects
ARABIDOPSIS thaliana; DROUGHT tolerance; ETHYLENE receptors; SOYBEAN; AMINO acid sequence; PLANTS
- Publication
Biologia Plantarum, 2013, Vol 57, Issue 1, p26
- ISSN
0006-3134
- Publication type
Article
- DOI
10.1007/s10535-012-0146-7