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- Title
A recycled batch biotransformation strategy for 22-hydroxy-23,24-bisnorchol-4-ene-3-one production from high concentration of phytosterols by mycobacterial resting cells.
- Authors
Hu, Yu; Wang, Da; Wang, Xuedong; Wei, Dongzhi
- Abstract
Objectives: To realize a practical technology for recycling both cyclodextrin and resting-cells at the same time in phytosterol biotransformation using mycobacterial resting cells. Results: In order to produce 22-hydroxy-23,24-bisnorchol-4-ene-3-one (HBC) efficiently and low-costly, a recycled phytosterols (PS) biotransformation process using mycobacterial resting cells was developed. By optimizing the ratio of hydroxypropyl-β-cyclodextrin (HP-β-CD) and PS to 1:1 (w/w), most products crystallized during the biotransformation process. So, the HBC was easily separated by low-speed (900×g) centrifugation with yield of 92%. The resting cells, HP-β-CD and the residual products and substrates left in the reaction system were reused for another bioconversion cycle after PS supplement. Three continuous cycles were achieved without the supplement of cells and HP-β-CD. In each batch, 80 g L−1 of PS was transformed to HBC with the space–time yield of HBC of 8.9–12.8 g L−1 day−1. Conclusions: This strategy reduced the cost of HBC production and simplified the purification process.
- Subjects
HUDSON'S Bay Co.; PHYTOSTEROLS; BIOCONVERSION; CELLS; INDUSTRIAL costs; CENTRIFUGATION
- Publication
Biotechnology Letters, 2020, Vol 42, Issue 12, p2589
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-020-02991-1