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- Title
PCI-24781 can improve in vitro and in vivo developmental capacity of pig somatic cell nuclear transfer embryos.
- Authors
Jin, Long; Zhu, Hai-Ying; Guo, Qing; Li, Xiao-Chen; Zhang, Yu-Chen; Zhang, Guang-Lei; Xing, Xiao-Xu; Xuan, Mei-Fu; Luo, Qi-Rong; Yin, Xi-Jun; Kang, Jin-Dan
- Abstract
Objective: To examine the effect of PCI-24781 (abexinostat) on the blastocyst formation rate in pig somatic cell nuclear transferred (SCNT) embryos and acetylation levels of the histone H3 lysine 9 and histone H4 lysine 12. Results: Treatment with 0.5 nM PCI-24781 for 6 h significantly improved the development of cloned embryos, in comparison to the control group (25.3 vs. 10.5 %, P < 0.05). Furthermore, PCI-24781 treatment led to elevated acetylation of H3K9 and H4K12. TUNEL assay and Hoechst 33342 staining revealed that the percentage of apoptotic cells in blastocysts was significantly lower in PCI-24781-treated SCNT embryos than in untreated embryos. Also, PCI-24781-treated embryos were transferred into three surrogate sows, one of whom became pregnant and two fetuses developed. Conclusion: PCI-24781 improves nuclear reprogramming and the developmental potential of pig SCNT embryos.
- Subjects
SOMATIC cell nuclear transfer; BLASTOCYST; ACETYLATION; LYSINE; LABORATORY swine
- Publication
Biotechnology Letters, 2016, Vol 38, Issue 9, p1433
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-016-2141-0