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- Title
Compressive mechanical stress may activate IKK-NF-κB through proinflammatory cytokines in MC3T3-E1 cells.
- Authors
Lu, Ying; Zheng, Qinghua; Lu, Wanlu; Yue, Junli; Zhou, Wei; Zhou, Xuedong; Zhang, Lan; Huang, Dingming
- Abstract
Objective: To determine whether IKK-NF-κB is activated either directly by compressive mechanical stress or by proinflammatory cytokines produced by MC3T3-E1 cells under compressive stress loading. Results: MC3T3-E1 cells subjected to cyclic uniaxial compressive stress showed increased expression of proinflammatory cytokines and activation of the IKK-NF-κB signaling pathway with nuclear translocation of p65. Following treatment with antibodies to neutralize the action of the proinflammatory cytokines, IL-1β and IL-6, the activation of IKK-NF-κB signaling was notably inhibited in MC3T3-E1 cells subjected to force loading. Conclusion: IKK-NF-κB signaling in MC3T3-E1 cells may be activated by proinflammatory cytokines that are produced as a consequence of mechanical stress loading and not by direct compressive mechanical stress.
- Subjects
IKAPPA B kinase; NF-kappa B; CELLULAR signal transduction; CYTOKINES; GENE expression
- Publication
Biotechnology Letters, 2015, Vol 37, Issue 9, p1729
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-015-1849-6