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- Title
Effect of α-tocopherol on the synthesis of phosphatidylglycerol catalyzed by phospholipase D in an aqueous system.
- Authors
Suzuri, Kounosuke; Yamamoto, Yukihiro; Hosokawa, Masashi; Miyashita, Kazuo
- Abstract
Phosphatidylglycerol (PG) was synthesized from several phosphatidylcholines (PCs) via phospholipase D (PLD)-catalyzed transphosphatidylation in an aqueous system. The yield of PG were 71 and 68 mol% from soybean PC and egg yolk PC, respectively, under the optimum reaction conditions of 50 μmol PC, 10 mmol glycerol, 3 ml of acetate buffer, 1.6 U PLD, and 30 μmol CaCl2 at 37°C for 48 h. In case of salmon roe PC with 14.3% eicosapentaenoic acid and 26.8% docosahexaenoic acid, the PG yield increased to 94 mol% by addition of 46 μmol α-tocopherol, although the PG yield was only 10% in absence of α-tocopherol.
- Subjects
PHOSPHOLIPASES; ESTERASES; SALMON; DOCOSAHEXAENOIC acid; OMEGA-3 fatty acids; GLYCERIN; ALCOHOLS (Chemical class); NITROGLYCERIN; EGG yolk
- Publication
Biotechnology Letters, 2009, Vol 31, Issue 5, p719
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-009-9929-0