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- Title
Amperometric Biosensors Based on Direct Electron Transfer Enzymes.
- Authors
Schachinger, Franziska; Chang, Hucheng; Scheiblbrandner, Stefan; Ludwig, Roland
- Abstract
The accurate determination of analyte concentrations with selective, fast, and robust methods is the key for process control, product analysis, environmental compliance, and medical applications. Enzyme-based biosensors meet these requirements to a high degree and can be operated with simple, cost efficient, and easy to use devices. This review focuses on enzymes capable of direct electron transfer (DET) to electrodes and also the electrode materials which can enable or enhance the DET type bioelectrocatalysis. It presents amperometric biosensors for the quantification of important medical, technical, and environmental analytes and it carves out the requirements for enzymes and electrode materials in DET-based third generation biosensors. This review critically surveys enzymes and biosensors for which DET has been reported. Single- or multi-cofactor enzymes featuring copper centers, hemes, FAD, FMN, or PQQ as prosthetic groups as well as fusion enzymes are presented. Nanomaterials, nanostructured electrodes, chemical surface modifications, and protein immobilization strategies are reviewed for their ability to support direct electrochemistry of enzymes. The combination of both biosensor elements—enzymes and electrodes—is evaluated by comparison of substrate specificity, current density, sensitivity, and the range of detection.
- Subjects
CHARGE exchange; BIOSENSORS; COPPER enzymes; ENZYMES; ENVIRONMENTAL compliance; ELECTROCATALYSIS; COFACTORS (Biochemistry)
- Publication
Molecules, 2021, Vol 26, Issue 15, p4525
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules26154525