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- Title
Modeling the dynamics of actin and myosin during bleb stabilization.
- Authors
Asante-Asamani, Emmanuel; Dalton, Mackenzie; Brazill, Derrick; Strychalski, Wanda
- Abstract
The actin cortex is very dynamic during the migration of eukaryotes. In cells that use blebs as leading-edge protrusions, the cortex reforms beneath the cell membrane (bleb cortex) and completely disassembles at the site of bleb initiation (actin scar). The molecular factors that regulate the dynamic reorganization of the cortex are not fully understood. Myosin motor protein activity has been shown to be necessary for blebbing, with its major role associated with pressure generation to drive bleb expansion. Here, we examine the role of myosin in regulating cortex dynamics during bleb stabilization. Analysis of microscopy data from protein localization experiments in Dictyostelium discoideum cells reveal a rapid formation of the bleb cortex with a delay in myosin accumulation. In the degrading actin scar, myosin is observed to accumulate before active degradation of the cortex begins. Through a combination of mathematical modeling and data fitting, we identify that myosin helps to regulate the equilibrium concentration of actin in the bleb cortex during its reformation by increasing its disassembly rate. Our analysis also suggests that cortex degradation in the actin scar is driven primarily by an exponential decrease in the actin assembly rate rather than increased myosin activity.
- Subjects
MOLECULAR motor proteins; ACTIN; MYOSIN; DICTYOSTELIUM discoideum; DATA analysis
- Publication
Applied Mathematics for Modern Challenges, 2024, Vol 2, Issue 1, p1
- ISSN
2994-7669
- Publication type
Article
- DOI
10.3934/ammc.2024002