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- Title
响应面优化畜禽归芪益母汤酶解提取工艺.
- Authors
刘雪松; 薛沾枚; 杨庆稳; 张艳; 孟维珊; 王爽; 陈曦; 杨旭东; 罗天瑶; 朱庆贺; 钟鹏
- Abstract
In order to obtain the best enzymatic extraction process of traditional Chinese veterinary drug livestock and poultry Guiqi Yimu decoction, single factor experiment combined with response surface methodology was carried to optimize the enzymatic extraction process. The conditions for measurement included enzymolysis time, temperature, and pH value. The mass concentration of calycosin-7-O-β-D-glucopyranoside and ferulic acid in the enzymatic hydrolysis extract of livestock and poultry Guiqi Yimu decoction was determined. The results showed that the optimal hydrolysis process of livestock and poultry Guiqi Yimu decoction was as follows: temperature was 50 ℃, extraction time was 9 h, pH value was 5. 0. Under these conditions, the mass concentration of calycosin-7-O-β-D-glucopyranoside was 0. 217 mg/mL and the mass concentration of ferulic acid was 0. 122 mg/mL, which were similar to the model predicted values. Compared with the traditional extraction method, the mass concentration of calycosin-7-O-β-D-glucopyranoside and ferulic acid in the livestock and poultry Guiqi Yimu decoction extracted by cellulase enzymatic hydrolysis increased by 48. 63% and 69. 52%, respectively.
- Subjects
RESPONSE surfaces (Statistics); POULTRY processing; VETERINARY drugs; POULTRY; LIVESTOCK
- Publication
Journal of Henan Agricultural Sciences, 2023, Vol 52, Issue 7, p125
- ISSN
1004-3268
- Publication type
Article
- DOI
10.15933/j.cnki.1004-3268.2023.07.013