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- Title
蚕豆耐盐相关基因VfHKT1;1的克隆、生物信息学分析及表达特性.
- Authors
樊有存; 张红岩; 杨旭升; 韩芊; 刘玉皎; 武学霞
- Abstract
High affinity K+ transporter (HKT) involve in salt tolerance response through maintaining Na+/K+ homeostasis in cellar and whole plant. VfHKT1;1 gene was cloned using RT-PCR and PCR in this work and the gene encoded an open reading frame (1 659 bp) consisting of 552 amino acids. Phylogenetic tree analysis indicated that VfHKT1;1 belong to HKT subfamily Ⅰ and shared the highest sequence similarity with MtHKT1;1, so it was named as VfHKT1;1. The bioinformatics analysis showed that VfHKT1;1 was stable, which also contained 8 transmembrane regions and no signal peptide. It was supposed to be an ion transporter which usually located at membrane because it contains a typical TrKH conserved functional domain of HKT family. Real-time fluorescent quantitative PCR showed different expression levels of VfHKT1;1 gene in root and leaf. The expression of VfHKT1;1 gene in root was suppressed under salt stress.Whereas it had higher transcript levels in leaf under salt stress, the expression peak appeared at 1 h after NaCl treatment. Then, the expression level of VfHKT1;1 gene gradually declined with the NaCl treatment time.
- Subjects
MOLECULAR cloning; PEPTIDES; GENE expression; AMINO acids; FAVA bean; EFFECT of salt on plants
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 4, p756
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.04.12