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- Title
Tracing Norovirus outbreak based on sequencing of regions coding for capsid proteins and RNA polymerase.
- Authors
HE Jiao-ming; YU Mu-hua; CHEN Hui; MA Yan-ping; ZHANG Hai-long; JU Chang-yan
- Abstract
Objective To analyze the etiological characteristics of an outbreak of Norovirus related to kitchen workers, in order to provide evidence for tracing norovirus infection. Methods The pathogen detection system of Filmarray was used for preliminary screening. Bacteria were isolated according to the diagnostic standard of infectious diarrhea WS 271-2007. Norovirus was also detected by fluorescent PCR. The regions coding for capsid proteins and RNA polymerase were amplified, sequenced and compared during Norovirus positive nucleic acids, and the homology was analyzed by Mega software. Results Among 49 patients, the positive rate of norovirus G I was 48.98%(24/49), G I was 2.04% (1/49), and the mixed positive rate of G I and G H was 2.04% (1/49). Among 115 samples of kitchen workers and related staff, the positive rate of Norovirus G H was 13.04%(15/115), G I was 0.87% (1/115). In 38 environmental samples, the positive rate of Norovirus G H was 2.63%(1 /38). The regions of Norovirus coding for capsid proteins and RNA polymerase was sequenced successfully in 17 samples, including 14 patients with G H type, 1 patient with G I type and 2 kitchen workers with G H type. G I subtype was G I .P4-G I .5, G H subtype was G H .P7-G H .6. The norovirus sequences of 2 Kitchen workers and 14 patients were 99% homologous. Among 49 patients, the positive rate of virulence gene of diarrheal Escherichia coli was 34.69% (17/49). There were 5 patients mix infected Norovirus and virulence gene of diarrheal Escherichia coli, the positive rate was 10.20%(5/49). In 16 positive norovirus samples of kitchen workers, the virulence gene of diarrheal Escherichia coli was not detected. The other samples were not tested for diarrheal Escherichia coli. Conclusions The outbreak is caused by norovirus G H .P7-G H .6 subtype. The patients and kitchen workers are likely to come from the same source of infection and are mix infected with diarrheal Escherichia coli. We should strengthen the physical examination and training of kitchen workers and related staff.
- Publication
China Tropical Medicine, 2022, Vol 22, Issue 1, p63
- ISSN
1009-9727
- Publication type
Article
- DOI
10.13604/j.cnki.46-1064/r.2022.01.14