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- Title
Early detection breast cancer: role of circulating plasma miRNA-21 expression as a potential screening biomarker.
- Authors
DIANSYAH, Muhammad Noor; PRAYOGO, Ami Ashariati; SEDANA, Made Putra; SAVITRI, Merlyna; ROMADHON, Pradana Zaky; AMRITA, Putu Niken Ayu; WIJAYA, Andi Yasmin; HENDRATA, Winona May; BINTORO, Ugroseno Yudho
- Abstract
Background/aim: To explore the potential of the circulating plasma miRNA-21 as an early detection biomarker by comparing early-stage breast cancer (BG) and healthy control (HG) in Indonesian population. Materials and methods: The enlisted patients were 26 adult female early-stage breast cancer patients (stage 1A, 1B, 2A, and 2B) of Airlangga University Hospital from August 2019 to October 2019. Sixteen volunteers were recruited as matching healthy subjects. MiRNA-21 expression was quantified by plasma qRT-PCR. Data analysis performed using IBM SPSS Statistics v.24 (IBM Corp., Armonk, NY, USA). MiRNA-21 cut-off, sensitivity, and specificity were analyzed using receiver operating characteristic (ROC) curve. Results: The study included 26 BG and 16 HG subjects. The miRNA-21 expression in BG group was 3.933 (1.181-11.794) and 0.905 (0.164-4.532) in HG group (4.34 folds; P = 0.001), with 1.66 cut-off (92.3% sensitivity; 81.2% specificity). MiRNA-21 expression separated analysis in HG showed a 0.578 times lower expression in menopause subjects [0.651 (0.164-0.414)], compared to premenopause ones [(1.123 (0.758 - 4.532); P = 0.031]. Yet, in BG group, 1.729 times higher miRNA-21 expression was observed in menopause subjects (6.021 ± 3.583), compared to premenopause ones (3.500 ± 1.517; P = 0.022). Conclusion: Circulating miRNA-21 expression is a potential biomarker for early detection of breast cancer and might act as a breast cancer risk predictor.
- Subjects
NEW York (N.Y.); EARLY detection of cancer; INTERNATIONAL Business Machines Corp.; RECEIVER operating characteristic curves; BIOMARKERS; VOLUNTEER recruitment; PERIMENOPAUSE; CLIMACTERIC
- Publication
Turkish Journal of Medical Sciences, 2021, Vol 51, Issue 2, p562
- ISSN
1300-0144
- Publication type
Article
- DOI
10.3906/sag-2005-138