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- Title
Arthrobotrys musiformis (Orbiliales) Kills Haemonchus contortus Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates.
- Authors
Pérez-Anzúrez, Gustavo; Olmedo-Juárez, Agustín; von-Son de Fernex, Elke; Alonso-Díaz, Miguel Ángel; Delgado-Núñez, Edgar Jesús; López-Arellano, María Eugenia; González-Cortázar, Manasés; Zamilpa, Alejandro; Ocampo-Gutierrez, Ana Yuridia; Paz-Silva, Adolfo; Mendoza-de Gives, Pedro
- Abstract
Haemonchus contortus (Hc) is a parasite affecting small ruminants worldwide. Arthrobotrys musiformis (Am) is a nematode-trapping fungi that captures, destroys and feeds on nematodes. This study assessed the predatory activity (PA) and nematocidal activity (NA) of liquid culture filtrates (LCF) of Am against Hc infective larvae (L3), and additionally, the mycochemical profile (MP) was performed. Fungal identification was achieved by traditional and molecular procedures. The PA of Am against HcL3 was performed in water agar plates. Means of non-predated larvae were recorded and compared with a control group without fungi. LCF/HcL3 interaction was performed using micro-tittering plates. Two media, Czapek–Dox broth (CDB) and sweet potato dextrose broth (SPDB) and three concentrations, were assessed. Lectures were performed after 48 h interaction. The means of alive and dead larvae were recorded and compared with proper negative controls. The PA assessment revealed 71.54% larval reduction (p < 0.01). The highest NA of LCF was found in CDB: 93.42, 73.02 and 51.61%, at 100, 50 and 25 mg/mL, respectively (p < 0.05). Alkaloids and saponins were identified in both media; meanwhile, coumarins were only identified in CDB. The NA was only found in CDB, but not in SPDB. Coumarins could be responsible for the NA.
- Subjects
HAEMONCHUS contortus; FUNGAL cultures; LARVAE; NEMATODE-destroying fungi; SWEET potatoes; AGAR plates
- Publication
Pathogens, 2022, Vol 11, Issue 10, p1068
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens11101068