We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Concurrent validity of biofilm detection by wound blotting on hard-to-heal wounds.
- Authors
Astrada, Adam; Nakagami, Gojiro; Minematsu, Takeo; Goto, Taichi; Kitamura, Aya; Mugita, Yuko; Sanada, Hiromi
- Abstract
Objective: Wound biofilms delay healing of hard-to-heal wounds. Convenient biofilm identification tools for clinical settings are currently not available, hindering biofilm-based wound management. Wound blotting with biofilm staining is a potential tool for biofilm detection, owing to its convenience. Although predictive validity of wound blotting has been established, it is necessary to confirm its concurrent validity. Furthermore, current staining systems employing ruthenium red have some disadvantages for clinical use. This study aimed to evaluate the usability of alcian blue as a substitute for ruthenium red. Method: Both in vitro and in vivo clinical samples were used to investigate validity and usability. Results: The in vitro study showed that proteins and extracellular DNA in biofilms did not affect staining ability of ruthenium red and alcian blue in the detection of biofilms. In the in vivo study, using a wound biofilm model with Pseudomonas aeruginosa, the staining sensitivity of ruthenium red was 88.9% and 100% for alcian blue, with correlation coefficients of signal intensities with native polyacrylamide gel electrophoresis (PAGE) of r=0.67 (p=0.035) and r=0.67 (p=0.036) for ruthenium red and alcian blue, respectively. Results from clinical samples were r=0.75 (p=0.001) for ruthenium red and r=0.77 (p<0.001) for alcian blue. The sensitivities of wound blotting staining by ruthenium red and alcian blue were very high and had a good correlation with native PAGE analysis. Conclusion: Because the alcian blue procedure is more convenient than the ruthenium red procedure, wound blotting with alcian blue staining would be a promising tool to guide clinicians in delivering biofilm-based wound management.
- Subjects
IN vitro studies; CHRONIC wounds &; injuries; INDOLE compounds; STAINS &; staining (Microscopy); IN vivo studies; PULSED-field gel electrophoresis; WOUND infections; RESEARCH methodology; RESEARCH methodology evaluation; ANIMAL experimentation; BIOFILMS; RATS; INORGANIC compounds; PSEUDOMONAS diseases; DESCRIPTIVE statistics; RESEARCH funding; DATA analysis software; STATISTICAL correlation; WOUND care
- Publication
Journal of Wound Care, 2021, Vol 30, Issue Sup4, pS4
- ISSN
0969-0700
- Publication type
Article
- DOI
10.12968/jowc.2021.30.Sup4.S4