We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Enhanced 5‐aminovalerate production in Escherichia coli from l‐lysine with ethanol and hydrogen peroxide addition.
- Authors
Cheng, Jie; Zhang, Yining; Huang, Meihua; Chen, Peng; Zhou, Xiaohua; Wang, Dan; Wang, Qinhong
- Abstract
BACKGROUND Increasing environmental and energy concerns are pushing the development of a bio‐based economy, employing highly reproducible, sustainable and green biomanufacturing methods. Here a potential building block 5‐aminovalerate for polymer synthesis has been produced from l‐lysine. RESULTS: l‐lysine α‐oxidase from Scomber japonicas was overexpressed in BL21(DE3); a lysine degradation gene was knocked out to strengthen this process in the microbe. The additions of ethanol and hydrogen peroxide significantly enhanced the production of 5‐aminovalerate. The recombinant Escherichia coli CJ02 strain was cultured in a medium containing 20 g L−1 glucose, 10 g L−1l‐lysine, 4% (v/v) ethanol and 10 mM H2O2, producing 5.61 g L−1 5‐aminovalerate with a yield of 0.56 g g−1. Compared with the original producer, this titre represents an 18‐fold increase. Excellently, 29.12 g L−1 5‐aminovalerate could be achieved with a yield of 0.44 g g−1 in a 5 L fermenter. CONCLUSION: This biotechnological 5‐aminovalerate production demonstrates a simple, economic, and green technology to replace the ubiquitous chemical synthesis. More importantly, this strategy of adding ethanol to increase protein expression is not only an efficient process for the production of 5‐aminovalerate, but also might be used in the production of other high value‐added chemicals. © 2018 Society of Chemical Industry
- Subjects
VALERATES; ESCHERICHIA coli; LYSINE; HYDROGEN peroxide; GREEN technology; FERMENTATION
- Publication
Journal of Chemical Technology & Biotechnology, 2018, Vol 93, Issue 12, p3492
- ISSN
0268-2575
- Publication type
Article
- DOI
10.1002/jctb.5708