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- Title
Identification and functional characterization of a sulfate transporter induced by both sulfur starvation and mycorrhiza formation in Lotus japonicus.
- Authors
Giovannetti, Marco; Tolosano, Matteo; Volpe, Veronica; Kopriva, Stanislav; Bonfante, Paola
- Abstract
Arbuscular mycorrhizas ( AMs) are one of the most widespread symbioses in the world. They allow plants to receive mineral nutrients from the symbiotic fungus which in turn gets back up to 20% of plant carbon and completes its life cycle. Especially in low-nutrient conditions, AM fungi are capable of significantly improving plant phosphate and nitrogen acquisition, but fewer data are available about sulfur ( S) nutrition., We focused on S metabolism in Lotus japonicus upon mycorrhizal colonization under sulfur starvation or repletion. We investigated both tissue sulfate concentrations and S-related gene expression, at cell-type or whole-organ level., Gene expression and sulfate tissue concentration showed that Rhizophagus irregularis colonization can improve plant S nutritional status under S starvation. A group 1 sulfate transporter, Lj Sultr1;2, induced by both S starvation and mycorrhiza formation, was identified. Its transcript was localized in arbuscule-containing cells, which was confirmed with a promoter- GUS assay, and its function was verified through phenotyping of TILLING mutants in nonmycorrhizal seedlings., Lj Sultr1;2 thus appears to encode a key protein involved in plant sulfate uptake. In contrast to phosphate transporters, a single gene, Lj Sultr1;2, seems to mediate both direct and symbiotic pathways of S uptake in L. japonicus.
- Subjects
VESICULAR-arbuscular mycorrhizas; MYCORRHIZAS; LOTUS japonicus; ENDOMYCORRHIZAS; SYMBIOSIS
- Publication
New Phytologist, 2014, Vol 204, Issue 3, p609
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.12949