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- Title
Novel and conventional assays in determining abundance of Streptococcus mutans in saliva.
- Authors
GAO, XIAO‐LI; SENEVIRATNE, CHAMINDA J.; LO, EDWARD C.M.; CHU, CHUN HUNG; SAMARANAYAKE, LAKSHMAN P.
- Abstract
International Journal of Paediatric Dentistry 2012; 22: 363-368 Background. Effective caries control and management requires identification of susceptible children for timely intervention. Streptococcus mutans ( S. mutans) is an important biomarker of caries risk. Aim. This study aimed to comparatively evaluate the validities of a novel immunoassay and a conventional culture-based assay in detecting salivary S. mutans in a paediatric cohort. Methods. 190 children aged 3-4 years were recruited. The abundance of S. mutans in their saliva samples was analysed with three assay systems viz. a conventional culture-based assay (Dentocult SM), a novel immunoassay system (Saliva-Check MUTANS) based on monoclonal antibody technology and a Taqman real-time PCR assay taken as a gold standard. Results. The novel immunoassay accurately differentiated saliva samples with high (≥5 × 105 CFU/mL) and low (<5 × 105 CFU/mL) S. mutans levels. The sensitivity/specificity was 97.6%/90.6%. The conventional culture-based assay reached a reasonably high sensitivity/specificity (92.8%/81.3%) in identifying children with moderate (≥104 CFU/mL) S. mutans level. Its sensitivity/ specificity in selecting children with high (≥105 CFU/mL) and very high (>106 CFU/mL) S. mutans levels were not sufficient (78.7%/79.8% and 25.8%/91.8%, respectively). Conclusion. The monoclonal antibody-based immunoassay accurately and rapidly determines S. mutans abundance in saliva and could be useful for chairside assessment of children's caries risk.
- Subjects
HONG Kong (China); DENTAL caries risk factors; SALIVA analysis; BACTERIAL growth; BIOMARKERS; IMMUNOASSAY; MICROBIOLOGICAL techniques; MONOCLONAL antibodies; PRESCHOOLS; POLYMERASE chain reaction; RESEARCH; STREPTOCOCCUS mutans; SAMPLE size (Statistics); STATISTICAL significance; HUMAN research subjects; PATIENT selection
- Publication
International Journal of Paediatric Dentistry, 2012, Vol 22, Issue 5, p363
- ISSN
0960-7439
- Publication type
Article
- DOI
10.1111/j.1365-263X.2011.01207.x