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- Title
Triggering neural differentiation of ES cells by subtype switching of importin-α.
- Authors
Yasuhara, Noriko; Shibazaki, Noriko; Tanaka, Shinya; Nagai, Masahiro; Kamikawa, Yasunao; Oe, Souichi; Asally, Munehiro; Kamachi, Yusuke; Kondoh, Hisato; Yoneda, Yoshihiro
- Abstract
Nuclear proteins are selectively imported into the nucleus by transport factors such as importin-α and importin-β. Here, we show that the expression of importin-α subtypes is strictly regulated during neural differentiation of mouse embryonic stem (ES) cells, and that the switching of importin-α subtype expression is critical for neural differentiation. Moreover, reproducing the switching of importin-α subtype expression in undifferentiated ES cells induced neural differentiation in the presence of leukaemia inhibitory factor (LIF) and serum, coordinated with the regulated expression of Oct3/4, Brn2 and SOX2, which are involved in ES–neural identity determination. These transcription factors were selectively imported into the nucleus by specific subtypes of importin-α. Thus, importin-α subtype switching has a major impact on cell differentiation through the regulated nuclear import of a specific set of transcription factors. This is the first study to propose that transport factors should be considered as major players in cell-fate determination.
- Subjects
NERVE tissue proteins; EMBRYONIC stem cells; CELL differentiation; TRANSCRIPTION factors; LEUKEMIA inhibitory factor; SERUM; GENE expression
- Publication
Nature Cell Biology, 2007, Vol 9, Issue 1, p72
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/ncb1521