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- Title
CLONING AND BIOLOGICAL ACTIVITY OF MURINE ONCOSTATIN M.
- Authors
HARA, Takahiko; ICHIHARA, Masatoshi; YOSHIMURA, Akihiko; MIYAJIMA, A.
- Abstract
Oncostatin M (OSM) is a member of the interleukin-6/leukemia inhibitory factor (LIF) family cytokines. While human OSM (hOSM) has been characterized, the murine counterpart had not been isolated. We cloned a routine OSM (mOSM) cDNA as a gene that is induced in hematopoietic cells by a subset of cytokines including IL-3, GM-CSF and Epo. Identity of mOSM was based on overall homology to hOSM and chromosomal gene localization. Human OSM is known to exhibit bioloiccal activities similar to LIF, because they share the same functional receptor composed of the LIF receptor and gpl30. As compared to hOSM, however, a 1000-fold higher concentrations of mOSM was required to stimulate proliferation of LIF-dependent murine DAla cells, differentiation of MI macrophage cells, and inhibition of ES cell differentiation. On the other hand, mOSM inhibited growth of NIH3T3 cells at a 1000-fold lower concentration than that of hOSM. These results indicate that mOSM functions through a receptor which is distinct from that of the LIF receptor. Studies on the physiological role of OSM is underway.
- Subjects
CLONING; INTERLEUKIN-6; CYTOKINES; ANTISENSE DNA; INTERLEUKIN-3; GRANULOCYTE-macrophage colony-stimulating factor; CELL differentiation
- Publication
Leukemia (08876924), 1997, Vol 11, p449
- ISSN
0887-6924
- Publication type
Article