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- Title
SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii.
- Authors
Salazar, Octavio R.; Chen, Ke; Melino, Vanessa J.; Reddy, Muppala P.; Hřibová, Eva; Čížková, Jana; Beránková, Denisa; Arciniegas Vega, Juan Pablo; Cáceres Leal, Lina María; Aranda, Manuel; Jaremko, Lukasz; Jaremko, Mariusz; Fedoroff, Nina V.; Tester, Mark; Schmöckel, Sandra M.
- Abstract
The identification of genes involved in salinity tolerance has primarily focused on model plants and crops. However, plants naturally adapted to highly saline environments offer valuable insights into tolerance to extreme salinity. Salicornia plants grow in coastal salt marshes, stimulated by NaCl. To understand this tolerance, we generated genome sequences of two Salicornia species and analyzed the transcriptomic and proteomic responses of Salicornia bigelovii to NaCl. Subcellular membrane proteomes reveal that SbiSOS1, a homolog of the well-known SALT-OVERLY-SENSITIVE 1 (SOS1) protein, appears to localize to the tonoplast, consistent with subcellular localization assays in tobacco. This neo-localized protein can pump Na+ into the vacuole, preventing toxicity in the cytosol. We further identify 11 proteins of interest, of which SbiSALTY, substantially improves yeast growth on saline media. Structural characterization using NMR identified it as an intrinsically disordered protein, localizing to the endoplasmic reticulum in planta, where it can interact with ribosomes and RNA, stabilizing or protecting them during salt stress. The authors show that in the halophyte Salicornia the sodium transporter SOS1 localizes to the tonoplast, likely storing sodium in the vacuole. The intrinsically disordered protein SALTY, increases yeast salt tolerance possibly stabilizing ribosomes in the ER.
- Subjects
SALINITY; SALT marshes; PROTEINS; ENDOPLASMIC reticulum; CROPS; EFFECT of salt on plants
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-48595-5