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- Title
Efecto de la radiación UV en la inactivación genética del esperma de botete diana Sphoeroides annulatus (Jenyns, 1842).
- Authors
Arias-Rodríguez, Lenin; Rodríguez-Ibarra, Luz Estela; del Valle-Pignataro, Gabriela
- Abstract
Genetic (DNA) inactivation of fish sperm with ultraviolet irradiation is generally accompanied by a paradoxical effect on survival rates (Hertwig effect). In the present study, sperm samples from ten male bullseye puffer fish (Sphoeroides annulatus) were diluted 1:50 using Cortland's extender solution and used to test the effect of nine ultraviolet doses (0.2-1.0 J cm-2) on motility time in seconds, motility index, and embryo survival rate after fertilizing eggs from five bullseye puffer females. Motility time of sperm irradiated with 0.2-0.9 J cm-2 were not statistically different from the controls, but sperm irradiated with a dosage of 1.0 J cm-2 dosage had significant lower motility time. Motility indices (MI) allowed for the statistical differentiation of four groups in relation to their response to different radiation doses: the first had high MI, and included the controls and 0.2-0.3 J cm-2 treatments; the second had lower MI and included the 0.4-0.7 J cm-2 treatments; the third showed recovery of MI and included the 0.8-0.9 J cm-2 treatments; and the fourth showed the lowest MI with the 1.0 J cm-2 treatment. Embryo survival was highest for the controls and 0.2 J cm-2 treatment, decreasing in the 0.3-0.4 J cm-2 treatments, increasing again in the 0.5-0.8 J cm-2 treatments, until reaching lowest survival in the 0.9-1.0 J cm-2 treatments. These results indicate that the best ultraviolet dosage to achieve genetic inactivation of sperm of this species is close to 0.7 J cm-2, a dosage in which fish fry showed typical haploid syndrome characteristics.
- Subjects
GENETICS; ULTRAVIOLET radiation; BULLSEYE puffer; EMBRYOS; SPERMATOZOA; NUCLEIC acids
- Publication
Ciencias Marinas, 2004, Vol 30, Issue 3, p391
- ISSN
0185-3880
- Publication type
Article
- DOI
10.7773/cm.v30i3.334