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- Title
Enzyme inhibitory, Antifungal, Antibacterial and hemolytic potential of various fractions of Colebrookia oppositifolia.
- Authors
Riaz, Tauheeda; Abbasi, Muhammad Athar; Aziz-ur-Rehman; Siddiqui, Sabahat Zahra; Shahid, Muhammad; Fatima, Hina; Ashraf, Muhammad; Ejaz, Syeda Abida; Rasool, Zahid Ghulam; Abbasi, Ghulam Hasan
- Abstract
The purpose of the present investigation was to assess the enzyme inhibition, antifungal, antibacterial and hemolytic activities of various fractions of Colebrookia oppositifolia Smith. The MeOH extract of plant was dissolved in dist. water and partitioned with n-hexane, CHCl3, EtOAc and n-BuOH sequentially. Enzyme inhibition studies were done against four enzymes i.e. α-glucosidase, butyrylcholinesterase, acetyl cholinesterase and lipoxygenase. Ethyl acetate fraction possessed very good activity against a-glucosidase (IC50 57.38±1.23µg/mL). CHCl3 fraction displayed good activity against a-glucosidase and lipoxygenase while moderate activity against butyryl cholinesterase. EtOAc fraction displayed good activity against lipoxygenase. Antifungal activity was studied against four fungi i.e. Aspergillus niger, Aspergillus flavus, Ganoderma lucidum and Alternaria alternata by the disc diffusion method using fluconazole, a standard antifungal drug, as positive control. Aqueous fraction displayed good activity against G. lucidum and A. flavus. Antibacterial activity was checked against Staphylococcus aureus, Bacillus subtilis, Pasturella multocida and Escherichia coli by the disc diffusion method using streptomycin sulphate, a standard antibiotic, as positive control. Chloroform, ethyl acetate and aqueous fraction showed good activity against E. coli. Chloroform fraction showed good activity against B. subtilis. Ethyl acetate fraction showed good activity against the P. multocida. All the studied fractions showed very less toxicity i.e. < 7%.
- Subjects
ENZYME inhibitors; ANTIFUNGAL agents; ANTIBACTERIAL agents; LAMIACEAE; THERAPEUTIC use of plant extracts
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2017, Vol 30, Issue 1, p105
- ISSN
1011-601X
- Publication type
Article