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- Title
Chromatographic methods for simultaneous determination of diiodohydroxyquinoline and Metronidazole in their binary mixture.
- Authors
Wageih Ali, Nouruddin; Gamal, Mohammed; Abdelkawy, Mohammed
- Abstract
Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline (DIHQ) and metronidazole (MTN). In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform: acetone: glacial acetic acid (7.5: 2.5: 0.1, by volume) as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water : methanol (60 :40, V/V, PH=3.6 )as mobile phase at a flow rate of 0.7 mL.min-1 and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation.
- Subjects
CHROMATOGRAPHIC analysis; HYDROXYQUINOLINE; METRONIDAZOLE; BINARY mixtures; DRUG development; SILICA gel; CHLOROFORM; THERAPEUTICS
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2013, Vol 26, Issue 5, p865
- ISSN
1011-601X
- Publication type
Article