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- Title
Reduced galactosyltransferase mRNA levels are associated with the agalactosyl IgG found in arthritis-prone MRL-<em>lpr/lpr</em> strain mice.
- Authors
Jeddi, P. A.; Lund, T.; Bodman, K. B.; Sumar, N.; Lydyard, P. M.; Pouncey, L.; Heath, L. S.; Kidd, V. J.; Delves, P. J.
- Abstract
MRL-lpr/lpr strain mice have defectively glycosylated IgG. This may be related to the rheumatoid arthritis (RA)-like disease that occurs in these mice, because a similar glycosylation defect is seen in human subjects with RA. Whilst it is known that this defect is associated with reduced activity of the β-l,4-galactosyltransferase (β-l,4-GalTase) enzyme, the cause of this reduced activity is at present unknown. We have therefore examined the molecular genetics of β-1,4-GalTase in MRLlpr/lpr mice. Using 10 different restriction endonucleases we found no evidence for a polymorphic variant of the gene in glycosylation-defective mice. However, the level of mRNA for β-1,4-GalTase was lowest in the MRL-lpr/lpr mice, the strain with the most poorly galactosylated IgG of the four strains examined. Thus, the reduced level of IgG oligosaccharide galactosylation found in MRLlpr/lpr strain mice appears to be related to either an altered transcriptional level of, or altered mRNA stability for, β-l,4-GalTase in lymphocytes from these mice.
- Subjects
IMMUNOGLOBULIN G; RHEUMATOID arthritis; GLYCOSYLATION; GALACTOSYLTRANSFERASES; IMMUNITY; IMMUNOLOGY
- Publication
Immunology, 1994, Vol 83, Issue 3, p484
- ISSN
0019-2805
- Publication type
Article