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- Title
Contribution of Extracellular and Intracellular Calcium to the Enhanced Effect of an alpha-Adrenergic Agonist on Amylase Release from Dispersed Rat Parotid Cells.
- Authors
TAKEMURA, H.; OHSHIKA, H.
- Abstract
The role of calcium in the potentiation of amylase release by the α-adrenergic agonist methoxamine (MTX) was examined using dispersed rat parotid acinar cells. The stimulatory effect of MTX (10µM) on the β-adrenergic agonist isoproterenol (ISP, 1 µM)-induced amylase release was blocked by the α-adrenergic antagonist phentolamine (10µM). In Ca-free (1 mMEGTA) medium, the increment of amylase release by MTX in normal medium was decreased by about 70%, but MTX still potentiated ISP-induced amylase release. MTX did not affect the cyclic AMP accumulation activated by ISP in either normal or Ca-free medium. MTX enhanced the ISP-stimulated uptake and efflux of 45Ca2+. These results suggest that both extracellular Ca2+ and intracellular stored Ca2+ may play an important role in the potentiation of amylase release from rat parotid acinar cells.
- Subjects
PHYSIOLOGICAL effects of calcium; PHENYLPROPANOLAMINE; AMYLASES; ANIMAL models in research; PAROTID glands; CELL metabolism; LABORATORY rats
- Publication
Journal of Dental Research, 1985, Vol 64, Issue 6, p881
- ISSN
0022-0345
- Publication type
Article
- DOI
10.1177/00220345850640060401