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- Title
Extraction, partial purification and determination of some biochemical properties of β-glucosidase from Tea Leaves ( Camellia sinensis L.).
- Authors
Şener, Aysun
- Abstract
This research was carried out to determine biochemical properties of β-glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21) isolated from Turkish tea leaves. Two protein peaks containing β-glucosidase activity were recovered and characterized, which were denoted as isoenzyme A and isoenzyme B. Their pH optimum, thermal resistances, affinity towards p-nitrophenyl-β-D-glucopyranoside differed markedly. They both displayed maximal activity at pH 5.0. The effects of the inhibitors tested varied in a dose dependent manner.
- Publication
Journal of Food Science & Technology, 2015, Vol 52, Issue 12, p8322
- ISSN
0022-1155
- Publication type
Article
- DOI
10.1007/s13197-015-1915-z