We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Recovery of Rutin from Labisia pumila Extract Using Solid Phase Extraction.
- Authors
Lee Suan Chua; Ruzlan, Nur Nabihah; Sarmidi, Mohamad Roji
- Abstract
Reflux extraction was used to prepare crude extract from the leaves of Labisia pumila var. Alata using 60% methanol. The crude extract was subsequently fractionated by C18 solid phase extraction to recover high yield of rutin using 20-100% methanol. The volume of eluent to recover rutin was found to decrease with the increase of methanol concentration. The recovery of rutin was increased from 20 to 80% methanol system, but slightly decreased in the 100% methanol system. Approximately, 70% of rutin could be recovered using the 80% methanol system. This solvent system also appears to have the lowest distance (9.44 MPa1/2) for rutin as estimated by Hansen solubility. The recovered rutin rich fraction could achieve up to 3.96 mg/g of fraction which was about 4-fold increment from the crude extract. The increment was also noticed for its antioxidant capacity expressed as scavenging activity which was 2 times higher than crude extract. A portion of water (20%) in the 80% methanol system is important to improve the yield of rutin. Rutin is a glycosylated flavonol, and therefore a small portion of water could enhance its elution compared to the lower performance of 100% methanol in rutin recovery.
- Subjects
PLANT extracts; EXTRACTION (Chemistry); METHANOL; GLYCOSYLATED hemoglobin; SOLVENTS
- Publication
Acta Chimica Slovenica, 2017, Vol 64, Issue 4, p888
- ISSN
1318-0207
- Publication type
Article
- DOI
10.17344/acsi.2017.3500