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- Title
Bioproduction of 4-Vinylphenol and 4-Vinylguaiacol β-Primeverosides Using Transformed Bamboo Cells Expressing Bacterial Phenolic Acid Decarboxylase.
- Authors
Kitaoka, Naoki; Nomura, Taiji; Ogita, Shinjiro; Kato, Yasuo
- Abstract
Phenolic acid decarboxylase (PAD) catalyzes the decarboxylation of hydroxycinnamic acids to produce hydroxystyrenes, which serve as starting materials for the production of polymers. Bamboo (Phyllostachys nigra; Pn) cells, a suitable host for producing phenylpropanoid-derived compounds, were transformed to express PAD of Bacillus amyloliquefaciens (BaPAD). BaPAD-transformed cells accumulated several metabolites that were not detected in wild-type Pn cells or BaPAD-negative transformant. Two major metabolites were isolated from BaPAD-transformed cells, and elucidation of their chemical structures confirmed these as 4-vinylphenol β-primeveroside (4-VPP) and 4-vinylguaiacol β-primeveroside (4-VGP). The production titers of 4-VPP and 4-VGP reached 48 and 33 mg/L at the maximum, respectively. Feeding experiments with 4-vinylphenol (4-VP), 4-vinylguaiacol (4-VG), and their glucosides indicated that 4-VPP and 4-VGP are formed by sequential glycosylation of 4-VP and 4-VG via their corresponding glucosides. Our results demonstrate the versatility of Pn cells for producing styrene derivatives, and indicate the presence of a unique glycosylation pathway to produce 4-VPP and 4-VGP in Pn cells.
- Subjects
PHENOLIC acids; BACTERIAL cells; STYRENE derivatives; BACILLUS amyloliquefaciens; HYDROXYCINNAMIC acids; BAMBOO; GLUCOSIDES
- Publication
Applied Biochemistry & Biotechnology, 2021, Vol 193, Issue 7, p2061
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-021-03522-y