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- Title
Bacteriophage-Resistant Salmonella rissen : An In Vitro Mitigated Inflammatory Response.
- Authors
Capparelli, Rosanna; Cuomo, Paola; Papaianni, Marina; Pagano, Cristina; Montone, Angela Michela Immacolata; Ricciardelli, Annarita; Iannelli, Domenico
- Abstract
Non-typhoid Salmonella (NTS) represents one of the major causes of foodborne diseases, which are made worse by the increasing emergence of antibiotic resistance. Thus, NTS are a significant and common public health concern. The purpose of this study is to investigate whether selection for phage-resistance alters bacterial phenotype, making this approach suitable for candidate vaccine preparation. We therefore compared two strains of Salmonella enterica serovar Rissen: RR (the phage-resistant strain) and RW (the phage-sensitive strain) in order to investigate a potential cost associated with the bacterium virulence. We tested the ability of both RR and RW to infect phagocytic and non-phagocytic cell lines, the activity of virulence factors associated with the main Type-3 secretory system (T3SS), as well as the canonic inflammatory mediators. The mutant RR strain—compared to the wildtype RW strain—induced in the host a weaker innate immune response. We suggest that the mitigated inflammatory response very likely is due to structural modifications of the lipopolysaccharide (LPS). Our results indicate that phage-resistance might be exploited as a means for the development of LPS-based antibacterial vaccines.
- Subjects
INFLAMMATION; FOODBORNE diseases; SALMONELLA; SALMONELLA enterica; INFLAMMATORY mediators; VIRULENCE of bacteria; SALMONELLA typhimurium
- Publication
Viruses (1999-4915), 2021, Vol 13, Issue 12, p2468
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v13122468