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- Title
Femtosecond and picosecond dynamics of recombinant bacteriorhodopsin primary reactions compared to the native protein in trimeric and monomeric forms.
- Authors
Smitienko, O.; Nekrasova, O.; Kudriavtsev, A.; Yakovleva, M.; Shelaev, I.; Gostev, F.; Dolgikh, D.; Kolchugina, I.; Nadtochenko, V.; Kirpichnikov, M.; Feldman, T.; Ostrovsky, M.
- Abstract
Photochemical reaction dynamics of the primary events in recombinant bacteriorhodopsin (bR) was studied by femtosecond laser absorption spectroscopy with 25-fs time resolution. bR was produced in an Escherichia coli expression system. Since bR was prepared in a DMPC-CHAPS micelle system in the monomeric form, its comparison with trimeric and monomeric forms of the native bacteriorhodopsin (bR and bR, respectively) was carried out. We found that bR intermediate I (excited state of bR) was formed in the range of 100 fs, as in the case of bR and bR. Further processes, namely the decay of the excited state I and the formation of intermediates J and K of bR, occurred more slowly compared to bR, but similarly to bR. The lifetime of intermediate I, judging from the signal of Δ A (470-480 nm), was 0.68 ps (78%) and 4.4 ps (22%) for bR, 0.52 ps (73%) and 1.7 ps (27%) for bR, and 0.45 ps (90%) and 1.75 ps (10%) for bR. The formation time of intermediate K, judging from the signal of Δ A (625-635 nm), was 13.5 ps for bR, 9.8 ps for bR, and 4.3 ps for bR. In addition, there was a decrease in the photoreaction efficiency of bR and bR as seen by a decrease in absorbance in the differential spectrum of the intermediate K by ~14%. Since photochemical properties of bR are similar to those of the monomeric form of the native protein, bR and its mutants can be considered as a basis for further studies of the mechanism of bacteriorhodopsin functioning.
- Subjects
BACTERIORHODOPSIN; RECOMBINANT proteins; CHEMICAL reactions; FEMTOSECOND lasers; PHOTOCHEMISTRY; LASER spectroscopy
- Publication
Biochemistry (00062979), 2017, Vol 82, Issue 4, p490
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297917040113