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- Title
Phenotypical and Genotypical Analysis of the Trueperella bernardiae Strain Isolated from a Dog.
- Authors
Arnafia, Wyanda; Ningrum, Siti Gusti; Sammra, Osama; Alssahen, Mazen; Wickhorst, Jörn-Peter; Lämmler, Christoph; Prenger-Berninghoff, Ellen; Timke, Markus; Abdulmawjood, Amir
- Abstract
The present study was designed to characterize phenotypically and genotypically the Trueperella bernardiae strain (T. bernardiae P5459/15) isolated from the purulent thelitis of a dog. The species identity could be confirmed by phenotypical investigations, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, and sequencing various molecular targets. Sequencing the 16S rDNA, the β subunit of bacterial RNA polymerase encoding gene rpoBand, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap revealed sequence similarities to the type strain T. bernardiae DSM 9152 of 99.6%, 99.3% and 99.1%, respectively. The present study is the first phenotypic and genotypic characterization of the T. bernardiae strain isolated from a dog.
- Subjects
GENETIC regulation; GENE expression in bacteria; DEHYDROGENASE genetics; DEHYDROGENASES regulation; DOG genetics; BACTERIA
- Publication
Veterinaria, 2017, Vol 66, Issue 2, p66
- ISSN
0372-6827
- Publication type
Article