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- Title
The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency.
- Authors
Bakirbas, Ahmet; Castro-Rodriguez, Rosario; Walker, Elsbeth L.
- Abstract
In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size—less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5′ tRFs and 5′ tRNA halves, are highly abundant in phloem sap, comprising about 46% of all reads. In addition, a set of miRNAs that are present in phloem sap was defined, and several miRNAs and sRNAs were identified that are differentially expressed during iron deficiency.
- Subjects
NON-coding RNA; IRON deficiency; PHLOEM; PLANT exudates; TRANSFER RNA; ARABIDOPSIS thaliana; RIBOSOMAL RNA
- Publication
Plants (2223-7747), 2023, Vol 12, Issue 15, p2782
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants12152782