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- Title
MiR-222 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting ETS1.
- Authors
SUN, Q.; JIANG, C.-W.; TAN, Z.-H.; HOU, L.-Y.; DONG, H.; LIU, K.; SUN, G.; LIU, Y.-J.; WANG, Y.-Q.; LU, X.-C.; LIU, H.
- Abstract
OBJECTIVE: The lung adenocarcinoma is a type of lung cancer. This research is to investigate the effects of miR-222 on the proliferation, migration and invasion of the lung adenocarcinoma cells. MATERIALS AND METHODS: At the beginning, MiR-222 and the controls were transfected to the lung adenocarcinoma cell line A549 for CCK-8 proliferation, transwell migration and Matrigel invasion, and then observed the effect of miR-222 on the proliferation, migration and invasion of lung adenocarcinoma cells. The miR- 222 target was regulated by ETS1 downwards to participate in the regulation of the process by using the luciferase reporter assay, the Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and the Western blotting. RESULTS: According to CCK-8 proliferation assay, the Transwell migration and the Matrigel invasion assay, it discovered that MiR-222 can promote the proliferation, migration and invasion of the lung adenocarcinoma cells. Luciferase reporter assay, RT-qPCR and Western blot assay showed that miR-222 could regulate the expression of ETS1 downwards and ETS1 participated in the regulation of the process CONCLUSIONS: ETS1 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting the regulated miR-222 downwards.
- Subjects
ADENOCARCINOMA; WESTERN immunoblotting; CELL lines; CYTOPROTECTION; REJUVENESCENCE (Botany)
- Publication
European Review for Medical & Pharmacological Sciences, 2017, Vol 21, Issue 10, p2385
- ISSN
1128-3602
- Publication type
Article