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- Title
Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins.
- Authors
Park, Jongjin; Yoon, Jiyoung; Kwon, Daekee; Han, Mi-Jung; Choi, Sunmee; Park, Slki; Lee, Junghyuk; Lee, Kiwook; Lee, Jaehwan; Lee, Seunghee; Kang, Kyung-Sun; Choe, Sunghwa
- Abstract
Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3′ exonuclease Recombination J (RecJ) or with GFP and demonstrated that transfection of the pre-assembled ribonucleoprotein of the two chimeric proteins into human or plant cells resulted in greater targeted mutagenesis efficiency up to 600% without noticeable increase in off-target effects. Improved activity of the two fusion proteins should enable editing of the previously hard-to-edit genes and thus readily obtaining the cells with designer traits.
- Subjects
GENOME editing; CRISPRS; CHIMERIC proteins; EXONUCLEASES; NUCLEOPROTEINS
- Publication
Scientific Reports, 2021, Vol 11, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-021-95406-8