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- Title
Immune recognition of the surface associated antigen, Tc-SAA-1, from infective larvae of Teladorsagia circumcincta.
- Authors
NISBET, A. J.; KNOX, D. P.; McNAIR, C. M.; MEIKLE, L. I.; SMITH, S. K.; WILDBLOOD, L. A.; MATTHEWS, J. B.
- Abstract
A cDNA encoding a surface-associated antigen was amplified by reverse transcriptase polymerase chain reaction (PCR) from RNA extracted from Teladorsagia circumcincta exsheathed third stage larvae (xL3). The protein encoded by this cDNA, Tc -SAA-1, displays 77% identity over 162 amino acid residues to a surface associated antigen from Ancylostoma caninum (Ac- SAA-1). Antiserum raised against a bacterially-expressed recombinant form of Tc -SAA-1 reacted with a native protein in somatic and surface extracts of xL3 but not with L4 or adult parasites. Limited binding of anti SAA-1 antibody was observed on the cuticular surface of xL3 s, however, regions of localization underlying the cuticle were observed. Incubation of xL3 T. circumcincta with anti-SAA rabbit serum failed to significantly inhibit penetration of the abomasal mucosa in vitro. IgA in abomasal mucus derived from sheep that had received a trickle infection of T. circumcincta bound recombinant Tc -SAA-1.
- Subjects
VETERINARY immunoparasitology; IMMUNE response; IMMUNOLOGY; ANTIGENS; POLYMERASE chain reaction
- Publication
Parasite Immunology, 2009, Vol 31, Issue 1, p32
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1111/j.1365-3024.2008.01070.x