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- Title
Impaired Somatodendritic Responses to Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) of Supraoptic Neurones in PACAP type I -Receptor Deficient Mice.
- Authors
Jamen, F.; Alonso, G.; Shibuya, I.; Widmer, H.; Vacher, C.-M.; Calas, A.; Bockaert, J.; Brabet, P.; Dayanithi, G.
- Abstract
Abstract The role of pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC1 receptor-deficient male mice (PAC1 -/- ). The effects of PACAP on [Ca2+ ]i were investigated in freshly dissociated supraoptic neurones and on the somatodendritic release of vasopressin and oxytocin, examined on intact supraoptic nuclei. In supraoptic neurones from wild-type mice (PAC1 +/+ ), 100 nm PACAP induced an increase in [Ca2+ ]i and release of vasopressin and oxytocin, whereas in heterozygous (PAC1 +/- ) and null-mutant mice (PAC1 -/- ), PACAP was much less effective. PACAP had no effect on these two parameters when applied to isolated neurohypophysial nerve terminals of PAC1 +/+ and PAC1 -/- mice, and rats. In conclusion, the PAC1 receptor is solely responsible for the PACAP-induced [Ca2+ ]i signalling and secretion of vasopressin and oxytocin in the somatodendritic region of supraoptic neurones.
- Subjects
PEPTIDE hormones; ADENYLATE cyclase; PITUITARY gland; DENDRITIC cells
- Publication
Journal of Neuroendocrinology, 2003, Vol 15, Issue 9, p871
- ISSN
0953-8194
- Publication type
Article
- DOI
10.1046/j.1365-2826.2003.01075.x