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- Title
The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer.
- Authors
Chen, T.‐H.; Chang, S.‐W.; Huang, C.‐C.; Wang, K.‐L.; Yeh, K.‐T.; Liu, C.‐N.; Lee, H.; Lin, C.‐C.; Cheng, Y.‐W.
- Abstract
Aim Colorectal cancer ( CRC) is the second commonest cause of cancer death in Taiwan. Although numerous genes have been associated with tumorigenesis in colorectal cancer, only a few have been validated and used as biomarkers for predicting clinical outcome. The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients. Method In total, 195 colorectal cancer patients were enrolled in a single medical centre between 2003 and 2007. APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction. The primary outcome included 5-year overall survival and univariate (Kaplan-Meier) and multivariate (Cox regression) analyses of prognostic factors. Results The results showed that 66 (33.8%) of 195 tumour tissues contained an APC mutation. The predominant APC gene variations were deletion mutations (50.0%). APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation. In advanced-stage cancer, patients with APC mutation/high miR-21 had poorer overall survival rates than those with APC mutation/low miR-21, APC wild-type/high miR-21 and APC wild-type/low miR-21. Conclusion In Taiwan, downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation. APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC, especially in patients with advanced disease.
- Subjects
TAIWAN; GENETICS of colon cancer; GENE expression; NEOPLASTIC cell transformation; HEALTH outcome assessment; MULTIVARIATE analysis
- Publication
Colorectal Disease, 2013, Vol 15, Issue 11, p1367
- ISSN
1462-8910
- Publication type
Article
- DOI
10.1111/codi.12318