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- Title
N-type Ca<sup>2+</sup> channels carry the largest current: implications for nanodomains and transmitter release.
- Authors
Weber, Alexander M.; Wong, Fiona K.; Tufford, Adele R.; Schlichter, Lyanne C.; Matveev, Victor; Stanley, Elise F.
- Abstract
Presynaptic terminals favor intermediate-conductance CaV2.2 (N type) over high-conductance CaV1 (L type) channels for single-channel, Ca2+ nanodomain-triggered synaptic vesicle fusion. However, the standard CaV1>CaV2>CaV3 conductance hierarchy is based on recordings using nonphysiological divalent ion concentrations. We found that, with physiological Ca2+ gradients, the hierarchy was CaV2.2>CaV1>CaV3. Mathematical modeling predicts that the CaV2.2 Ca2+ nanodomain, which is ∼25% more extensive than that generated by CaV1, can activate a calcium-fusion sensor located on the proximal face of the synaptic vesicle.
- Subjects
SYNAPTIC vesicles; ORGANELLES; SYNAPSES; NEUROSCIENCES; CELL nuclei
- Publication
Nature Neuroscience, 2010, Vol 13, Issue 11, p1348
- ISSN
1097-6256
- Publication type
Article
- DOI
10.1038/nn.2657