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- Title
Anti‐androgenic, anti‐oxidant and anti‐apoptotic effects of the aqueous and methanol extracts of Pterorhachis zenkeri (Meliaceae): Evidence from in vivo and in vitro studies.
- Authors
Bonsou Fozin, Georges Romeo; Deeh Defo, Patrick Brice; Wankeu‐Nya, Modeste; Ngadjui, Esther; Kamanyi, Albert; Watcho, Pierre
- Abstract
The aim of this study was to evaluate the effects of Pterorhachis zenkeri (Meliaceae) on sex organ growth in immature male rats and, oxidative stress and apoptosis markers in CCL‐97 (R2C) Leydig cells. For the in vivo studies, 70 immature male Wistar rats (n = 10/group) were treated for 2 or 4 weeks with: distilled water (10 ml/kg, per os) plus soya oil (1 ml/kg, sc), bicalutamide (10 mg/kg, per os), aqueous or methanol extract of P. zenkeri (10 mg/kg or 62 mg/kg, per os) or testosterone propionate (3 mg/kg, sc). After each treatment period, body and sexual organ weights, plasmatic testosterone, total proteins and total cholesterol levels were measured. In the in vitro test, the effects of the methanol extract of P. zenkeri on cell viability, apoptosis, reactive oxygen species (ROS) production, intracellular calcium release and caspases 3/9 were assessed using CCL‐97 Leydig cells. Pterorhachis zenkeri extracts decreased sex organ weights, plasmatic testosterone and protein levels in rats. In the in vitro studies, P. zenkeri inhibited apoptosis, ROS production, calcium release and caspase 3/9 activities. These results suggest that P. zenkeri has anti‐androgenic, anti‐oxidant and anti‐apoptotic activities with methanol extract being the most active and could be an effective alternative for the management of androgen‐related diseases.
- Subjects
REACTIVE oxygen species; GENITALIA; LEYDIG cells; SOY oil; IN vitro studies
- Publication
Andrologia, 2020, Vol 52, Issue 11, p1
- ISSN
0303-4569
- Publication type
Article
- DOI
10.1111/and.13815