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- Title
Biochemical Characterisation of α-Amylase in Two Aphid Species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae).
- Authors
DARVISHZADEH, ALI; BANDANI, ALIREZA; MOUSAVI, SEYED QASEM
- Abstract
We identify and characterise α-amylases of the two aphid species, A. fabae and A. gossypi. To do this, α-amylases of the two insect species were extracted and their activities were determined using 1% soluble starch. Results showed that α-amylase, which hydrolyses starch, is present in both aphids. Also, it was shown that optimum pH and temperature for the α-amylases of both species is 7.0 and 40°C, respectively. Gel assays using zymogram analysis showed that in both aphid species more than one isoform (two isoforms) of α-amylases hydrolyszing carbohydrates are present.
- Subjects
AMYLASES; APHIDS; CLASSIFICATION of insects; HYDROLYSIS; BEAN aphid; COTTON aphid
- Publication
Plant Protection Science, 2014, Vol 50, Issue 2, p84
- ISSN
1212-2580
- Publication type
Article
- DOI
10.17221/71/2012-pps