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- Title
The cAMP Receptor Protein (CRP) of Vibrio mimicus Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence.
- Authors
Tian, Ziqi; Xiang, Fei; Peng, Kun; Qin, Zhenyang; Feng, Yang; Huang, Bowen; Ouyang, Ping; Huang, Xiaoli; Chen, Defang; Lai, Weimin; Geng, Yi
- Abstract
Simple Summary: It is known that in highly pathogenic infections, the cAMP receptor protein (CRP) frequently plays an essential regulatory role. A highly pathogenic strain of Vibrio mimicus SCCF01 has been isolated from yellow catfish. To investigate the role of the cAMP receptor protein in regulating SCCF01, we created a strain with a deleted crp gene (Δcrp). The results demonstrated that the expression of genes related to the bacterial type II secretion system, flagellin, adhesion, and metalloproteinase was decreased by the deletion crp gene. The above resulted in modifications to the morphology of the bacteria and colonies, as well as a decrease in the motility, hemolytic activity, biofilm formation, bacterial growth, and enzyme activity. Animal experiments and cytotoxicity analyses verified that crp played a role in V. mimicus pathogenicity. In conclusion, these findings clarified the biological role of the crp gene in V. mimicus, revealed the pathogenic mechanism of the microorganism, and provided a basis for effective control and prevention of V. mimicus infection. Vibrio mimicus is a serious pathogen in aquatic animals, resulting in significant economic losses. The cAMP receptor protein (CRP) often acts as a central regulator in highly pathogenic pathogens. V. mimicus SCCF01 is a highly pathogenic strain isolated from yellow catfish; the crp gene deletion strain (Δcrp) was constructed by natural transformation to determine whether this deletion affects the virulence phenotypes. Their potential molecular connections were revealed by qRT-PCR analysis. Our results showed that the absence of the crp gene resulted in bacterial and colony morphological changes alongside decreases in bacterial growth, hemolytic activity, biofilm formation, enzymatic activity, motility, and cell adhesion. A cell cytotoxicity assay and animal experiments confirmed that crp contributes to V. mimicus pathogenicity, as the LD50 of the Δcrp strain was 73.1-fold lower compared to the WT strain. Moreover, qRT-PCR analysis revealed the inhibition of type II secretion system genes, flagellum genes, adhesion genes, and metalloproteinase genes in the deletion strain. This resulted in the virulence phenotype differences described above. Together, these data demonstrate that the crp gene plays a core regulatory role in V. mimicus virulence and pathogenicity.
- Subjects
PROTEIN receptors; QUORUM sensing; BACTERIAL growth; BACTERIAL adhesion; VIBRIO; FLATHEAD catfish; FLAGELLA (Microbiology)
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 3, p437
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14030437